B. Rita Alevriadou, Ph.D.
Associate Professor
Education
Undergraduate degree (Diploma of Chemical Engineering): Aristotle University, Thessaloniki, Greece, 1986
Graduate degree (M.S., Chemical Engineering): Rice University, Bioengineering & Biosciences Institute, Houston, TX, 1989
Graduate degree (Ph.D., Chemical Engineering): Rice University, Bioengineering & Biosciences Institute, Houston, TX, 1992
Professional Experience
Graduate Research Assistant, Department of Chemical Engineering, Bioengineering & Biosciences Institute, Rice University, Houston, TX; Advisor: Larry V. McIntire, Ph.D.; 1986-1992
Postdoctoral Fellow, Department of Molecular and Experimental Medicine, Committee on Vascular Biology, The Scripps Research Institute, La Jolla, CA; Supervisor: Zaverio M. Ruggeri, M.D.; 1992-1993
Assistant Professor, Department of Biomedical Engineering,
The Johns Hopkins University (JHU) School of Medicine, Baltimore, MD; 1993-2003;
Director, Vascular Bioengineering Laboratory;
Joint appointment with Department of Chemical Engineering
Associate Professor, Biomedical Engineering & Internal Medicine (Cardiology),
The Ohio State University (OSU), Columbus, OH; 2003-present;
Director, Vascular Bioengineering Laboratory;
Member, Davis Heart & Lung Research Institute (DHLRI);
Affiliated Member, Integrated Biomedical Science Graduate Program (IBGP);
Affiliated Member, Biophysics Graduate Program
Contact Information
The Ohio State University
473 West 12th Avenue, 610 DHLRI
Columbus, OH 43210
Tel. (614)292-5160
Fax: (614)292-8778 or (614)247-7799
Email: alevriadou-1@medctr.osu.edu
Laboratory: 665 DHLRI Tel. (614)688-4385
Alternate Office: 296 Bevis Tel. (614)292-8314
Affiliations
Member, Davis Heart & Lung Research Institute (DHLRI)
Affiliated Member, Integrated Biomedical Science Graduate Program (IBGP)
Affiliated Member, Biophysics Graduate Program
Lab Members
Michael P. Burns, Ph.D., Postdoctoral Fellow
Production of Reactive Oxygen Species (ROS) by Cultured Vascular Endothelial Cells (ECs) due to Ischemia/Reperfusion Treatment
Zhaosheng (John) Han, Ph.D., Postdoctoral Fellow
Effect of Ischemia/Reperfusion on EC Nitric Oxide Synthase (eNOS) Activation and Expression, and Nitric Oxide (NO) Production
Charles I. Jones III, M.S., Graduate Research Assistant
Effect of Ischemia/Reperfusion on EC Antioxidant Enzyme Activities
Guru Meenakshisundaram, MS, BME PhD candidate, Graduate Research Assistant
Role of EC Redox Status on Expression of Adhesion Molecules for Leukocytes
Area of expertise
Vascular biology/bioengineering, endothelial cell mechanotransduction (signal transduction due to mechanical forces), oxidative stress, antioxidants, molecular mechanisms of blood cell adhesion, thrombosis, inflammation
Research Interests
- Role of hemodynamic forces (in particular, fluid shear stress) and/or oxidative stress in vascular endothelial cell (EC) signal transduction, gene expression and protein synthesis
- Role of hemodynamic forces, blood cell receptors and plasma proteins (in particular, fibrinogen and vWF) in thrombosis (platelet adhesion/aggregation) and inflammation (leukocyte-EC adhesion)
- Design of in vitro systems to mimic the hemodynamic environment in vascular health and disease (eg. to simulate the EC dysfunction after ischemia/reperfusion or heart transplantation)
Current efforts concentrate on investigating the role of endogenous ROS in EC dysfunction following ischemia/reperfusion (I/RP) by using an in vitro experimental model that allows exposure of flow-preconditioned ECs to changes in both fluid flow (shear stress) and oxygen tension. Our data provide quantitative information on how these changes, either each one acting separately or both together (as in the case of I/RP), affect the EC redox status (ROS production and status of antioxidant enzymes), nitric oxide (NO) release and endothelial constitutive nitric oxide synthase (eNOS) phosphorylation/activation (funded by NIH).
Teaching
BME 721 (Fall Quarter) "BIOLOGICAL TRANSPORT" (co-taught with
Dr. Nic Moldovan)
Course Description: It is an advanced undergraduate/graduate
course. Its objective is to present engineering fundamentals and
biological applications in a unified way. It covers topics in
fluid mechanics, mass transport and biochemical interactions with
engineering concepts motivated by biological problems.
BME 694 (Spring Quarter) "FUNDAMENTALS OF CELL & TISSUE
ENGINEERING" (co-taught with Dr. Doug Kniss)
Course Description: It is an introduction to cell and tissue
engineering principles with emphasis on cell function (adhesion,
biomaterial interactions, migration, metabolism), tissue
development and growth (tissue repair, angiogenesis,
inflammation), and tissue engineering approaches (tissue
induction, stem cells, gene therapy).
Recent publications/presentations
- M.T. Hinds, Y.J. Park, S.A. Jones, D.P. Giddens and B.R. Alevriadou, "Local hemodynamics affect monocytic cell adhesion to a three-dimensional flow model coated with E-selectin", J Biomech 34:95-103, 2001.
- T.C. Huang, R.E. Jordan, R.R. Hantgan and B.R. Alevriadou, "Differential effects of c7E3 Fab on thrombus formation and rt-PA-mediated thrombolysis under flow conditions", Thromb Res 102(5):411-25, 2001.
- L.H. Yeh, A.M. Kinsey, S. Chatterjee and B.R. Alevriadou, "Lactosylceramide mediates shear-induced endothelial superoxide production and intercellular adhesion molecule-1 expression", J Vasc Res 38(6):551-9, 2001.
- D.M. Wootton, A.S. Popel and B.R. Alevriadou, "An experimental and theoretical study on the dissolution of mural fibrin clots by tissue-type plasminogen activator", Biotechnol Bioeng 77(4):405-19, 2002.
- C.K.D. Ng, S.S. Deshpande, K. Irani and B.R. Alevriadou, "Adhesion of flowing monocytes to hypoxia/reoxygenation-exposed endothelial cells: role of Rac1, ROS and VCAM-1", Am J Physiol 283:C93-C102, 2002.
- K.V. Vijayan, T.C. Huang, Y. Liu, A. Bernardo, J.F. Dong, P.J. Goldschmidt-Clermont, B.R. Alevriadou and P.F. Bray, "Shear stress augments the enhanced adhesive phenotype of cells expressing the Pro33 isoform of integrin β3", FEBS Lett 540:41-6, 2003.
- B.R. Alevriadou, "CAMs and Rho small GTPases: gatekeepers for leukocyte transendothelial migration. Focus on: VCAM-1-mediated Rac signaling controls endothelial cell-cell contacts and leukocyte transmigration", Am J Physiol 285: C250-2, 2003.
- J.B. Haun, W.M. Baldwin III and B.R. Alevriadou, "Clearance of complement by human vascular endothelial cells: Effects of hypoxia/reoxygenation and IL-1β activation", Transpl Int 18:475-482, 2005.
- S.F. Martin, S. Chatterjee, N. Parinandi and B.R. Alevriadou, "Rac1 inhibition protects against hypoxia/reoxygenation-induced lipid peroxidation in human vascular endothelial cells", Vascul Pharmacol, 43(3):148-156, 2005.
